Part:BBa_K726001:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K726001

Through massive literature investigation, NJMU-China found that serotonin acts as an interkingdom signaling molecule via quorum sensing. Specifically, no response was noted in the rhl cellular system, indicating specificity for the las QS pathway. Our part consists of a sensor sequence: lasI Promoter, and a fusion protein sequence: lasI ORF + linker + lacZ, which not only serves as a reporter that will turn the culture medium blue within the presence of Qurom-Sensing molecules (QSMs) or proteins alike, but also starts a positive feedback that enhances the transcriptional activity of our responsive promoter. It could be used to design biosensors that aim to detect QSMs and show a visible color change.

We evaluated the docking scores between the complex of LasR (a signaling molecule in las QS pathway) - Serotonin/HSL (QS molecule in las QS pathway) and PlasI, and found that serotonin has similar interaction patterns with HSL compared with the negative control.

As a preliminary characterization and verification of our parts, we simulated the interactions between proteins (LasR, LacZ), DNA (PlasI) and small molecules (Serotonin, Dopamine, 3-O-C12-HSL, X-gal). We employed homology modeling to predict protein structures from SWISS-MODEL, and constructed the DNA structure. Also, we simulated and visualized the docking through Hdock and Pymol and verified the function of our parts at the modeling dimension.

User Reviews

UNIQ89107497ef19d620-partinfo-00000000-QINU UNIQ89107497ef19d620-partinfo-00000001-QINU